What are the necessary equipment for the construction of microbiology laboratory?

It depends on what kind of experiment you do, generally large equipment: microscope, centrifuge, electrophoresis, ultraviolet projector, analytical balance, pipette, 4° freezer, -20 freezer, -80 freezer, shaker, incubator, shaker, electric stove.

Let's not talk about the little things, what else is decided according to the strength of the laboratory and the direction of the experiment.

Equipment for microbial pretreatment: balances, sterilizers, electric stoves, refrigerators of various temperatures, pipettes, electric incubators, shakers, media configurators, water purifiers.

Equipment for aseptic operation: ultra-clean bench or biosafety cabinet, sterile drenching facility, infrared sterilizer, sterilizable pipette, homogenizer.

Microbial culture equipment: biochemical incubators, anaerobic incubators, shakers, etc.

Other microbial experimental equipment: microscope, centrifuge, electrophoresis instrument, colony counter, microplate reader, gel imaging system, ultraviolet spectrophotometer, automatic microbial detection system, etc.

There should be a centrifuge, autoclave, incubator, microscope, shaker, pipette, electric stove, fermenter, alcohol lamp, petri dish, UV projector...Let's do it!

Not to mention the reagents, the necessary equipment is a microscope*, centrifuge, pipette, refrigerator, incubator*, shaker, electric stove, fermenter. (depending on the use) I hope it will be helpful to you!!

The commonly used instruments and equipment in the microbiology laboratory include anaerobic incubators, constant temperature incubators, dry heat sterilization boxes, autoclaves, and ultra-clean workbenches.

1. Anaerobic incubator.

Anaerobic incubators are also known as anaerobic workstations or anaerobic glove boxes. An anaerobic incubator is a special device for bacterial cultivation and operation in an anaerobic environment. It can provide strict anaerobic state, constant temperature incubation conditions and has a systematic and scientific working area.

2. Constant temperature incubator.

Thermostatic incubator is a general term for a type of thermostatic chamber. It is widely used in medical and health care, pharmaceutical industry, and biochemistry.

Scientific research departments such as industrial production and agricultural science are mainly responsible for cultivating various microorganisms or organisms such as tissues and cells.

3. Dry heat sterilization box.

The dry heat sterilization box adopts the dry heat sterilization method, which refers to the method of sterilization with high temperature dry hot air. This method is suitable for glass and metal products that are resistant to high temperatures and do not allow damp heat.

Sterilization of gas-penetrating oils and fats (such as oil-based ointment mechanisms, oils for injection, etc.) and high-temperature-resistant powder chemicals, suitable for high-temperature resistant glassware, porcelain, and glass syringes.

It is not suitable for the sterilization of rubber, plastic and most drugs.

4. Autoclave.

Autoclave is a device that uses saturated pressure steam to quickly and reliably sterilize items, which is suitable for medical and health, scientific research, agriculture and other units. Autoclaves are divided into portable autoclaves, vertical autoclaves, and horizontal autoclaves according to their styles and sizes. It can be used for medical devices, dressings, glassware, solution media.

and so on.

5. Ultra-clean workbench.

The ultra-clean workbench, also known as the purification workbench, is to adapt to modern industry, optoelectronic industry, and biopharmaceutical.

and scientific research, testing and other fields for the cleanliness of local work areas designed.

It draws air through a fan into the pre-filter and through a hydrostatic pressure box.

Enter the HEPA filter.

Filtration, the filtered air is sent out in the state of vertical or horizontal airflow, so that the operation area can reach the 100-level cleanliness and ensure the requirements of environmental cleanliness for production.

1. Ultra-low temperature freezer: cryopreservation of vaccines, strains, biological samples, etc.

2. Liquid nitrogen tank: long-term active preservation of vaccines, bacteria and viruses, and cells.

3. Drug refrigerator: used for refrigerated storage of drugs, reagents, biological products and vaccines.

General large-scale equipment: microscope, centrifuge, electrophoresis instrument, ultraviolet projector, analytical balance, pipette, 4° ice high pickpocket, -20 refrigerator, -80 refrigerator, shaker, incubator, shaker, electric stove.

Yes, it must be approved. But it doesn't have to be. If it is a university laboratory, it must be approved by the Ministry of Education. If it is a provincial-level department, then it must be approved. Not the same.

A microbiology laboratory is a place where microbial research is conducted. According to the safety requirements and use requirements of the microbiology laboratory, it should be different from the general laboratory engineering or purification engineering. Laboratories mainly used in microbiology, biomedicine, biochemistry, animal experiments, genetic recombination, and biological products are collectively referred to as biosafety laboratories.

The biosafety laboratory is composed of the main functional laboratory, other laboratories and auxiliary functional rooms. The biosafety laboratory must ensure the safety of people, the environment, the waste and the samples, and operate safely for a long time, while also providing a comfortable and good working environment for the laboratory staff.

Preparation room: The preparation room is used for the preparation of culture media and sample processing, etc. The room is equipped with reagent cabinets, counters for storing utensils or materials, laboratory benches, electric stoves, refrigerators, water and sewerage, power supplies, etc.

Sterilization room: The sterilization room is mainly used for the sterilization of culture medium and the sterilization of various utensils, and the room should be equipped with high-pressure steam sterilizer, oven and other sterilization equipment and facilities.

Sterile room: The sterile room, also known as the inoculation room, is a special laboratory for aseptic operations such as systematic inoculation and purification of strains. In microbial work, inoculation and transplantation of strains is a major operation, which is characterized by ensuring that the strains are pure, and preventing contamination by miscellaneous bacteria.

In the air of the general environment, due to the presence of a lot of dust and miscellaneous bacteria, it is easy to cause pollution, which greatly interferes with the inoculation work.

Washing room: The washing room is used for scrubbing utensils, etc. Pathogenic microorganisms are sometimes present due to microbial contamination of used utensils.

Therefore, if conditions permit, zui-good set up the washing chamber. The room should be equipped with heaters, steamers, basins and buckets for washing utensils, as well as various bottle brushes, decontamination powder, soap, washing powder, etc.

The microbiology laboratory is mainly responsible for the detection of various microbial items, including the total number of colonies, the type of colonies, pathogenic coocci, etc. According to different classifications, microbiology laboratories have different safety requirements and use requirements, so microbiology laboratories are different from the construction concept of general laboratory (purification) projects.

The basic requirements for the construction of the microbial testing laboratory are that the surface of the wall must be smooth, waterproof and corrosion-resistant, the ground must be leak-free, smooth but not slippery, and the furnishings of the instruments and equipment are simple, easy to clean, easy to disinfect and clean, so that the experimental environment can be kept (clean) and sterile as much as possible. The basic furniture of the microbiological testing laboratory includes: laboratory bench, ultra-clean workbench, biological safety cabinet, etc.

In order to facilitate cleaning and cleaning, it is recommended to choose an isolated ** laboratory bench, which has a functional area dry and wet isolation module, which can be moisture-proof and resistant to Li Wei corrosion, and the dry and wet areas are separated, making the experiment safer. The better isolated ** test bench should also be equipped with a full-station water and electricity supply function to flexibly meet the experimental needs; In order to facilitate access to reagents, you can also make full use of the space to design the stereo reagent storage space.

In addition, in order to provide a better experimental environment for microbial detection experiments and ensure the accuracy of experimental results, the laboratory has extremely high requirements for the cleanliness of the environment.