Can cell culture plates grow bacteria

Cell culture media:

Zhejiang Weikang brand cell culture medium includes MEM medium, DMEM medium, DMEM F12 medium, RPMI1640 medium, MEM medium, IMDM medium, M199 medium, and MCCOY'S 5A medium, HAM'S F-10 medium, HAM'S F-12 medium, HAM'S F-12K medium and Leibovitz'SL-15 medium, etc.

2.Disposable ready-made sterile medium with less nutrient loss and ready to use right out of the bottle.

3.Verified by multiplex cell testing, the culture quality is reliable, and the variability between batches is small.

4.In stock**, a variety of component combination cell culture media can be customized according to customer needs.

5.Filter membrane filtration sterilization, no pollution; Formulated with water for injection and low endotoxin.

Zhejiang Weikang Medical Technology Co., Ltd. is committed to developing a high-quality, effective and stable basic cell culture medium, providing suitable pH, osmotic pressure and nutrients for cell growth, so that cells can maintain a good state in an artificial simulation environment in vitro. Cell basal medium series products include MEM medium, DMEM medium, DMEM F12 medium, RPMI1640 medium, MEM medium, IMDM medium, M199 medium, and MCCOY'S 5A medium, HAM'S F-10 medium, HAM'S F-12 medium, HAM'S F-12K medium and Leibovitz'SL-15 medium, as well as a variety of customizable media, are suitable for in vitro culture of a variety of suspended and adherent mammalian cells and cell lines.

Yes, but it's overkill, the cell culture plate is more expensive, and the surface of the cell culture plate is treated.

Of course, if you can raise cells, you will definitely be able to raise bacteria. But it's too high, it's a bit of a waste!

Do not use round Petri dishes for bacterial culture.

When bacteria are cultured in petri dishes, they are used for surface culture of bacteria, using a solid medium.

The petri dish is sterilized first, usually wrapped and sterilized by dry heat at about 140 hours, and the time is 18 to 24 hours.

The medium is then prepared. The most common is complete medium, which is made of beef paste, peptone, glucose, NaCl, and sometimes a little magnesium sulfate or ferrous sulfate. Dissolve and mix these raw materials in water in turn according to the required amount, then add agar according to the amount, put it into a larger Erlenmeyer flask after boiling, plug the bottle mouth with a tampon tightly, put it into an autoclave for autoclaving, let it cool to about 40, pour it into the petri dish, cover the dish, and let it cool after the medium solidifies into a solid state, which can be used to cultivate bacteria.

Put the bacteria to be cultured or use the inoculation needle (inoculation loop) to insert the strain, or use a pipette to drop the liquid culture bacterial solution into the petri dish, coat it with a triangular glass spatula, cover the dish, and then put it into the constant temperature incubator for culture. After 24 48 hours, the bacterial growth and colony characteristics were observed.

After doing the experiment, how to deal with the medium of the petri dish for culturing the bacteria.

Hello, I'm honored to answer for you: If you are using a commercial disposable medium plate, then directly after use, it can be sterilized by high temperature and high pressure (121 15-30 minutes), and it can be disposed of according to general medical waste after disinfection. If it needs to be reused, then it also needs to be sterilized by high temperature and high pressure, and then it can be poured while the agar is in a liquid state and then cleaned.

Cell culture. Plate mold mu is used as a biological tool. Different shapes of cell culture plates are crack-resistant, and different materials are used for different purposes. Today's cell culture vessels, petri dishes, culture flasks, and culture plates in the hall are mostly made of polystyrene.

Material: Cell membrane.

Generally negatively charged, polystyrene coating is positively charged, which is used to promote cell adhesion. Polystyrene is a colorless and transparent thermoplastic with a glass transition temperature of more than 100 °C, and is colorless, transparent, with excellent optical properties and high rigidity.

The method of culturing bacteria in a petri dish is:

1. Clean the petri dish, wrap it in kraft paper or gauze, and sterilize it in an autoclave for 121 minutes for 30 minutes.

2. Dissolve the sample with sterile normal saline, inject 1ml of the diluted solution into the Petri dish, pour the sterilized medium, and wait for coagulation.

3. Place the petri dish in a 36 biochemical incubator for 2-3 days to observe the colonies growing in the medium.

A petri dish is a type of labware used for microbial or cell culture that consists of a flat disc-shaped bottom and a lid, generally made of glass or plastic. The material of petri dishes is basically divided into two categories, mainly plastic and silver glass, glass can be used for plant materials, microbial culture and adherent culture of animal cells. The plastic may be made of polyethylene material, with disposable reed banquets and multiple uses, which are suitable for laboratory inoculation, scribing, and bacterial isolation operations, and can be used for the cultivation of plant materials.

According to the purpose of culturing bacteria and the characteristics of the culture, the culture method can be divided into three types: general culture method, carbon dioxide culture method and anaerobic culture method.

1. General culture method: the inoculated medium is placed in the 37 incubator for 18 24 hours, and aerobic bacteria and facultative anaerobic bacteria can grow on the medium. A small number of slow-growing bacteria need to be cultured for 3 to 7 days until a month to grow.

To maintain a certain humidity in the incubator, a glass of water can be placed in it. For medium with a long culture time, the test tube mouth should be tamponned after inoculation and sealed with paraffin petroleum jelly to prevent the medium from drying out.

2. Carbon dioxide culture method: some bacteria, such as Brucella bovine abortus and Vibrio foetus, need to be in the air containing 10 carbon dioxide to grow, especially the primary isolation and culture requirements are more stringent. The method of placing the inoculated medium in the carbon dioxide environment for culture is the carbon dioxide culture method, 3. Anaerobic culture method

There are three commonly used anaerobic culture methods: anaerobic tank method, air bag method and anaerobic box.

Bacteria cannot be cultivated without a culture medium.

Cultivating bacteria does not necessarily require the use of medium liquid, but also can be solid and semi-solid substances, mainly depending on the metabolism mode of the cell, and the culture method is different for different metabolic methods.

Data Extensions. Culture medium refers to the nutrient matrix prepared by the combination of different nutrients for the growth and reproduction of microorganisms, plants or animals (or tissues). Generally, it contains carbohydrates, nitrogenous substances, inorganic salts (including trace elements), vitamins and water.

The medium is not only the basic material that provides cell nutrition and promotes cell proliferation, but also the living environment for cell growth and reproduction. There are many types of culture medium, which can be divided into natural medium, synthetic medium and semi-synthetic medium according to the preparation of raw materials; According to the physical state, it can be divided into solid medium, liquid medium and semi-solid medium; According to the culture function, it can be divided into basic medium, selection medium, enrichment medium, identification medium, etc. According to the scope of use of cavity masking, it can be divided into bacterial culture medium, actinomycete medium, yeast culture medium, fungal elimination medium, etc. After the medium is prepared, the pH is usually tested and adjusted, and sterilization, usually autoclaved and filter-sterilized.

Culture media are susceptible to contamination or deterioration due to their nutrient content. It should not be left for a long time after matching, and it is best to use it now.