Similarities and differences between plant pathogenic fungi and bacterial isolation techniques Urgen

Iso: 1Experimental Materials:

PDA Plate (Fungus) NA Plate (Bacteria) 2Experimental Methods: Tissue Isolation Method (Fungi) Scribing Separation Method (Bacteria) 3

After sterilization water cleaning, the filter paper absorbs water (fungus) and does not absorb water to make a suspension (bacteria) with: all are taken from the junction of the sick and healthy, 70 alcohol rinsing, 5 naclo disinfection, sterilized water cleaning, the operation is about the same, always maintain aseptic operation, and finally all are cultured in the 25-28 degree incubator.

I don't understand the question, what to isolate, isolate bacteria and fungi? Or isolate bacteria and plants.

The purpose of isolation is to clarify the class of the pathogen.

Commonly used surface disinfectantsSpecial isolation methods are all selective enrichment of pathogenic bacteria to be isolated.

The rules for writing Latin state that:

Plant pathogens are all parasites and are divided into the following according to lifestyle:

If the attachment cell enters directly through the orifice of the plant, it does not need to form an infection nail and then invade.

Comparison of Rhizopus and Mucor spp.

Infection with the outer cyst fungus, causing peach leaf shrinkage disease.

Origin of Ectocysts: Extocysts belong to the phylum Ascomycetes, and the ascospores of most fungi in the phylum Ascomycetes are produced in the ascomycetes. However, the ectocysts are more special, and the ascomycetes formed by the ectocysts are not produced in the ascomycetes, but are arranged on the surface of the host plant cells, so they are called ectocysts.

Powdery mildew fungi mainly cause powdery mildew in grapes, including leaves and fruits.

The reason for the separation of Powdery mildew from Powdery mildew spp. is that the appendage filaments of the closed capsule shell in the fungus are very short, while the Brinella is larger.

3. Gaeumanomyces causes total erosion of wheat, and the roots and stems appear black.

Fourth, the scab is a genus of sail dismantling.

Summary. Hello, the common methods for isolating plant pathogenic bacteria are isolation and culture method, screening method, biological characteristics separation method, immunological separation method, etc., these methods are suitable for different types of pathogens, isolation and culture method is suitable for the isolation of bacteria, fungi and other microorganisms The commonly used isolation medium is ordinary nutrient agar medium, selective agar medium, etc., the screening method is suitable for isolating variants or hybrid strains in pathogenic bacteria, and the commonly used screening methods are antibiotic screening method, fluorescence screening method, etc., The biological characteristics isolation method is suitable for isolating pathogenic bacteria with special biological characteristics<>

There are several commonly used methods for isolating plant pathogens, and these methods are suitable for which type of pathogens to isolate and how to isolate them.

Hello, the common methods for isolating plant pathogenic bacteria are isolation and culture method, screening method, biological characteristics separation method, immunological separation method, etc., these methods are suitable for different types of pathogens, isolation culture method is suitable for the isolation of bacteria, fungi and other microorganisms The commonly used isolation medium is ordinary nutrient agar medium, selective agar medium, etc., the screening method is suitable for isolating variants or hybrid strains in pathogenic bacteria, and the commonly used screening methods are antibiotic screening and manual modification method, fluorescent sieve potato pants selection method, etc., The biological characteristics isolation method is suitable for isolating pathogenic bacteria with special biological characteristics<>

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Fungal isolation and culture is a process in which clinical test specimens are separated and inoculated or transplanted in fungal culture medium, and placed in a suitable growth environment for incubation to obtain pure fungal species. Fungal reproduction can be both sexual reproduction and asexual spore reproduction, which can grow in artificial culture medium under suitable conditions and produce various forms of spores and asexual fruiting bodies, which is the main basis for fungal classification and identification.

Cultivation method: 1The culture media include solid agar, liquid medium, and two-way medium.

2.The main culture methods are test tube method, plate culture and slide culture.

3.The treated clinical specimens and laboratory-preserved strains were inoculated in 2 3 tubes of Shabaoweak agar inclined medium containing penicillin and streptomycin respectively, and the clinical specimens were directly inoculated, and the fungal strains were inoculated with sterilized inoculation loops according to the inclined medium inoculation method.

4.After inoculation, the medium was incubated in a 28 or 37 incubator, and the morphology and color changes of the colony were observed day by day.

The purpose of fungal isolation and culture is to detect fungi, further identify fungal species and do fungal drug susceptibility test, so as to provide a basis for the diagnosis and development of clinical fungal infectious diseases.

1. Experimental principle.

Fungal mycelium in diseased tissues of plants, if given suitable environmental conditions, are generally fine except for a few species.

Restore growth and reproduction. The isolation of plant pathogenic bacteria refers to the removal of pathogenic fungi from infected plant tissues through artificial culture.

It is separated from other miscellaneous bacteria, isolated from the host plant, and then the isolated pathogenic bacteria are purified in a suitable environment, which is generally called the isolation and culture of plant pathogens. The isolation of plant pathogenic fungi is generally carried out by tissue segregation, that is.

Small pieces of diseased tissue were cut off, washed with surface disinfection and sterilized water, and then moved to artificial medium for culture.

Second, the purpose of the experiment:

The isolation and culture of plant pathogens is one of the most basic operating techniques for plant pathology experiments.

The observation of the original morphology and the culture of plant disease inoculum are all commonly used research methods. Through this experiment, it is required.

General principles and methods for the isolation and culture of plant pathogens.

The isolation of pathogenic fungi is mainly carried out for the following purposes:

1. Provide a basis for the etiological diagnosis of diseases;

2. Guide clinical **;

3. Used for epidemiological investigation.

Because of the poor nutritional conditions of PDA medium, most bacteria cannot grow normally, but there are still a small number of bacteria that can grow.

Isolate unknown bacteria, because the bacteria are not sure which one they are, and some bacteria have different metabolic conditions and nutritional requirements, so it is difficult to say in general. In the case of clinical specimens, a highly nutrient medium such as a blood plate or chocolate plate (as well as fungi) should be used to ensure that the target bacteria can be isolated as much as possible, an anaerobic environment should be provided if anaerobic infection is suspected, and a special medium should be provided to culture it if specific bacteria such as Mycobacterium tuberculosis are suspected.

In addition, sometimes the culture program is set in a certain direction, such as testing a batch of food for Salmonella, then a selective medium for that bacterium should be used.

Fungal culture medium, such as Martin's medium, Cha's medium, potato medium. Yeast and bacteria can be easily eliminated due to the large mold colonies in solid cultures. Prokaryotic antibiotics such as streptomycin can be added to inhibit bacteria.

Wort medium is used for yeast separation, which generally eliminates mold. In the event of mold growth, the mold hyphae can be removed by filtration and then cultured to obtain yeast.