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At 100x magnification, the number of microns of the scale is generally about 100 microns. This value is calculated by measuring the length of the ruler in the microscope image and then from the known length of the ruler and the length of the image. When observing a sample, if you need to measure physical quantities such as length and width, you can calculate it based on the length of the ruler in the image and the number of microns of the ruler.
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At 500x magnification, the number of microns on the scale is generally about 20 microns. At this magnification, the microscope is able to observe finer details and structures, allowing for more precise measurements.
At 1000x magnification, the number of microns on the scale is generally about 10 microns. At this magnification, the microscope is able to observe even finer details and structures, allowing for more accurate measurements.
It is important to note that the magnification and scale accuracy of different brands and models of metallurgical microscopes will vary. When making measurements, it is necessary to select the appropriate instrument and ruler according to the specific experimental requirements, and perform the correct operation and calculation to obtain accurate experimental results.
If you have any needs, you can consult ZEISS microscopes. Zeiss microscope subordinate to the purchase of microscopes, recommend Beijing Presage Instruments****. With rich professional knowledge and many years of valuable experience in selling products, Beijing Presaisi Instrument has established a sales network with strong technical force and a wide range of products.
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It is a comparison ruler that represents the white line of the label.
The actual length is 50 microns, and the magnification can be calculated based on the length on **. Scale.
You can use this ruler to calculate how many times this image is magnified!
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100x microscope ruler marking: When taking pictures of the microscope, the shooting software supporting the microscope is generally used to add the ruler to the fixed position of **. In this way, the ruler is displayed.
In the process of taking pictures with a microscope, we usually use other shooting software to display the ruler in a fixed position. When displaying, it is often necessary to crop and typeset the **, and the ruler added to the ** is often cropped out.
The length of the rulerOn a given ruler, the length of the line segment between the first and last ruler marks through the midpoint of all the shortest marks. The line between the beginning and the end of the two ruler markers through the midpoint of all the shortest markers is called the ruler baseline, and the ruler length is the length of the ruler baseline. This line can be a curve or straight line of real or imaginary, and the length of the ruler is expressed in units of length, regardless of the unit being measured or marked on the scale.
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The 400x microscope ruler is 500x and 20um.
If the accuracy requirements are not high, just attach the ruler to the 400 times metallographic ** for a rough reference, if the requirements are fine, it is definitely necessary to calibrate. A C1 micrometer is required for calibration.
And although the objective lens of the microscope is said to be 40 times, this 40 times is certainly not exactly the same as the other 40 times magnification, there is an error, plus or minus, and the useful camera target is one-half, and one-third of the ** shot is not the same under the same objective.
When the ruler is bent.
In the neutral layer oo'The upper part is compressed, the size is shortened, the lower part is tensioned, the size becomes longer, and there is only the neutral layer OO'The length does not change. If the engraving is engraved on the upper or lower surface. Its dimensions are all changed, and only engraved on the neutral layer has the least impact on the size.
Therefore, the cross-section of the metal ruler is machined into a "U" or "H" shape, and the engraved line is engraved on the neutral layer.
Glass rulers, on the other hand, are difficult to process into complex shapes and are generally rectangular sections. According to the data, there are glass rulers processed into trapezoidal sections to reduce bending deformation, and there are also simple shape glue to synthesize "H" shapes, and the effect needs to be further studied.
The above content reference: Encyclopedia - Ruler.
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Generally, 1000um calibration is used, and the corresponding scale is 1000 50um; 1000/100um; 1000/200um; 1000/500um; 1000/1000um;i.e. 20um, 10um, 5um, 2um, 1um
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Microscope scale 300um is 33 times.
For example, if the scale is 200um, then the magnification is 50 times, and the scale is 500um, so the magnification is 20 times, so the scale is 20 times, so the scale is 300um, and the magnification is about 33 times.
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