What are the methods for rapidly culturing digestive bacteria?

Some nitrifying bacteria are added directly to the filter media, such as filter cotton, activated carbon, and porcelain rings are added. If you open the cylinder as new, pay attention to oxygenation.

The most direct way is to buy some nitrifying bacteria back, both liquid and solid, which are generally sold in ornamental fish stores.

Add water to the fish tank, if your fish tank has a filtration system, turn on the power of the filtration system and the oxygenation system, and pour the nitrifying bacteria you bought back directly on the filter material; If there is no filtration system, nitrifying bacteria are poured directly into the water. Generally, 10 liters of water with 5-10 ml of liquid nitrifying bacteria is enough, and generally you will have no problem putting the fish directly.

1) Configure the medium.

First of all, it is necessary to configure the medium containing nutrients, because the life of bacteria needs nutrients, not agar, which is a substance that can gelatin into solids after boiling and cooling (2) High temperature sterilization, sterilize the configured medium and Petri dish to prevent other bacteria from invading and affecting the experiment (3) Inoculate it after cooling (4) Cultivate it in an incubator, and the bacteria multiply faster in a place with a suitable temperature Note: Observe regularly and record in detail So the general steps for culturing bacteria and fungi in the laboratory are: preparation of culture medium - high temperature sterilization cooling - inoculation - culture So the answer is:

preparation of culture media; high temperature sterilization; cooling inoculation; Isothermal culture.

According to the purpose of culturing bacteria and the characteristics of the culture, the culture method can be divided into three types: general culture method, carbon dioxide culture method and anaerobic culture method.

1. General culture method: the inoculated medium is placed in the 37 incubator for 18 24 hours, and aerobic bacteria and facultative anaerobic bacteria can grow on the medium. A small number of slow-growing bacteria need to be cultured for 3 to 7 days until a month to grow.

To maintain a certain humidity in the incubator, a glass of water can be placed in it. For medium with a long culture time, the test tube mouth should be tamponned after inoculation and sealed with paraffin petroleum jelly to prevent the medium from drying out.

2. Carbon dioxide culture method: some bacteria, such as Brucella bovine abortus and Vibrio foetus, need to be in the air containing 10 carbon dioxide to grow, especially the primary isolation and culture requirements are more stringent. The method of placing the inoculated medium in a carbon dioxide environment for culture is the carbon dioxide culture method, 3. Anaerobic culture method: the commonly used anaerobic culture methods include anaerobic tank method, air bag method and anaerobic box.

The most comprehensive explanation of bacterial and fungal culture methods! Check it out.

The most comprehensive explanation of bacterial and fungal culture methods! Check it out.