How does microbial culture work

Principle:1If a single microorganism is too small, colony culture method is generally used for culture observation.

2.Due to the different growth environments of different microorganisms, there are clear boundaries between populations. A colony can be considered a collection of the same species.

Instruments: inoculation shovel and inoculation needle (for terrestrial cultures) Inoculation loop (for aquatic cultures) Alcohol lamp Petri dish.

Steps:1Prepare different concentrations of nutrient solution diluents: take 1ml of nutrient solution and dissolve it in 9ml of sterile water, shake for 5min to prepare 10% solution.

2.Bring the mouth of the container close (note not toating) the alcohol lamp flame and draw 1 ml from this solution with a pipette and add it to a tube containing 9 ml of sterile water. And so on to make dilutions of different concentrations such as 1%, , , etc.

3.Place the mouth of the test tube close to (note that it is not facing the flame of the alcohol lamp), hold the test tube in your left hand, and hold the petri dish in your right hand (only a small slit is exposed, and it is close to the flame lamp).Pour the solution into Petri dishes and label each dish with concentration.

4.Hold the mouth of the sample container with bacterial growth close to (note that it is not facing the alcohol lamp) and use the inoculation loop to pick a small amount of bacteria from the colony and add it to the 10% nutrient solution. The inoculation loop is burned on an alcohol lamp and sterilized, and the sample is again added to a Petri dish containing 1% nutrient solution (only a small slit is exposed, and it is close to the flame of the lamp).

Repeat this operation to seed the nutrient solution of each concentration.

5.Gently shake the Petri dish and store it in an incubator at 37 degrees Celsius, and colonies will appear after 2 to 3 days.

The most important thing: aseptic operation. The second is speed.

<> configure different concentrations of nutrient solution diluents; Extract the dilution (1 ml) and add it to a tube filled with 9 ml of sterile water, and so on to make dilutions of 1% and other different concentrations; Pour the solution into Petri dishes and mark the concentration on each Petri dish; Inoculate the nutrient solution at each concentration, then gently shake it to the Petri dish of the seeds, and then store it in an incubator (37°C).

Microorganisms generally do not have high requirements for nutrition, agricultural and sideline products, factories

Scraps can be used to cultivate microorganisms. Expert.

The biogas generator is to produce biogas from manure, plant fibers, etc. Most microorganisms have mild reaction conditions, can grow and multiply at room temperature and pressure, and do not require expensive equipment, which is much superior to the production of chemical raw materials by chemical methods. Microbial culture is not affected by the season or climate, so it can be industrialized for many years.