What mitochondria look like is visible under an electron microscope

What it looks like.

Chloroplasts and mitochondria were observed in high school biology experiments, which can be seen under the light microscope, but only an oval or rod-shaped shape can be seen, and the internal microstructure cannot be seen. Submicrostructure refers to the microstructures such as the bilayer membrane, sac-like structure, and crest that are depicted in textbooks seen under an electron microscope.

Ribosome. It's too small, and the magnification of an ordinary light microscope is not enough, so you can't see it, so you have to use an electron microscope.

And the Golgi apparatus.

It is a membrane structure, and if it is not stained, it cannot be seen, just like the endoplasmic reticulum, so it can only be seen after membrane staining.

Generally, cells are observed with a light microscope, and vacuoles and chloroplasts are the most obvious, because they both contain a certain amount of pigment. To see the nucleus.

Staining is also required. Mitochondria are also difficult to observe and generally require electron microscopy. As for the various membranes, since they are transparent, they must be dyed to be observed. (Electron microscope observation is different).

As shown in the figure, under the electron microscope, the mitochondria are a closed membranous sac structure composed of a double layer of unit membranes. The two membranes isolate the internal space of the mitochondria from the cytoplasm and separate the internal space of the mitochondria into two inner membranes and the outer membrane to form a scaffold for the mitochondria.

What mitochondria look like in the submicrostructure diagram of a cell.

The comrades upstairs have very different opinions, so let me say a few words.

You can see it!

Mitochondria are ellipsoidal organelles that are generally in diameter.

m, the length varies greatly, generally, and the resolution limit of an optical microscope is a micron, that is, 100 times the oil lens.

Now if you compare it yourself, even if a general microscope does not reach the limit resolution, you can still distinguish things that are 1 m, and the mitochondria are within the observation range, so you can definitely see them.

It's just that you can't see the internal structure, only some rugby-like grains.

After staining, it can be seen, but it is not clear, only a small dot-like structure, much smaller than the chloroplast of algae plants, and the structure of the inner membrane and the outer membrane cannot be distinguished, but it can be seen that it is a spherical body, which is unevenly distributed in the cytoplasm.

Scanning electron microscopy can only observe the surface of the placed sample, and the thickness of the surface signal is about 1 micron, so the mitochondria must be exposed with SEM observation.

TEM can only observe samples in the sub-micron (1 micron) range, so cells are sliced and mitochondria are cut out, or mitochondria are extracted directly.

Ultrathin sectioning: osmium acid fixation, alcohol dehydration, methyl methacrylate or epoxy embedding, final sectioning.

Cryo-etching: Rapid freezing of specimens, cutting with ice blades, and sublimation with platinum charcoal.

The thickness of the recognition surface signal is about 1 micron, so the mitochondria must be exposed with SEM observation 2, so the cells should be sectioned 1, the scanning electron microscope can only observe the surface of the sample put in, and the transmission electron microscope can only observe the submicron level (1 micron) thickness sample, and cut out the mitochondria.

Hello, dear, because the light microscope can make use of eyepieces and objective lenses to magnify cells. An optical microscope is an optical instrument that uses optical principles to magnify and image tiny objects that cannot be distinguished by the human eye, so that people can extract microstructural information. A microscope is a precision optical instrument that has been developed for more than 300 years.

Since the advent of microscopes, people have seen many tiny organisms and the basic unit cells that make up the cavity of the hide, which were not seen in the past. There are not only optical microscopes that can magnify more than 1,000 times, but also electron microscopes that magnify hundreds of thousands of times, which enable us to have a further understanding of the laws of life activities of living organisms. Most of the experiments specified in the biology syllabus of ordinary middle schools must be completed by microscopes, so the performance of the microscope is the key to doing a good job in observation experiments.

There are various organelles in cells, chloroplasts unique to plant cells, mitochondria, Golgi apparatus, ribosomes, etc., which are common in animal and plant cells...

1. Low-power optical microscope: you can see cells of general size, and they are very small, and generally only a circle of cell membranes can be seen (plant cells can also see the cell wall).

2. High-power optical microscope: you can see cells of general size, relatively large, and you can see large organelles such as nuclei (plant cells can also see large vacuoles and chloroplasts). What we see in an optical microscope is called microstructure.

Electron microscopy: You can see all the organelles in the cell, and even the approximate structure of the lipid bilayer of the biofilm (two black lines) can be seen under a good electron microscope, which we call the submicroscopic structure under the electron microscope.

Cells can be seen with a normal microscope! Generally use 400x or 1000x, you can see the cell wall, cell body, nucleus, etc.!

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Ordinary light microscope 1000x can see that the cell membrane has lysosomes, and the organelles other than ribosomes, of course, are all large eukaryotic cells.

Prokaryotic cells don't see anything unless they're moving fast enough to see movement, like Vibrio cholerae.

After staining, the basic morphology of the bacteria can be seen.

1. Low magnification: you can see cells of general size, and they are very small, and generally only a circle of cell membranes can be seen (plant cells can also see the cell wall).

2. High magnification: you can see cells of general size, relatively large, and you can see large organelles such as nuclei (plant cells can also see large vacuoles and chloroplasts).

What we see in an optical microscope is called microstructure.

Plants: cell wall, cell membrane (usually thin and not easily visible), cytoplasm, chloroplast, mitochondria, vacuoles, nucleus.

Animals: cell membrane, cytoplasm, nucleus.

In the cell there is a cell membrane, cytoplasm (including organelles), nucleus. Prokaryotic cells do not have a nucleus, and plants have an extra cell wall.

Ordinary microscopes can see everything except ribosomes in the cell membrane and cytoplasm.

Organelles: cell membranes, nuclei, mitochondria, Golgi apparatus, etc., ordinary microscopes can only see the structure of cells, if you want to see the above organelles, you have to use special staining.

Your question feels ambiguous.

1.Are you asking "why can't microsomes be observed by electron microscopy"? The answer is: microsomes can be seen under an electron microscope, such as in the figure below.

2.Did you prepare a sample yourself that may contain microsomes, but then no microsomes were observed under the electron microscope? Then there are too many possibilities, and there may be problems in all aspects of your sampling, sample preparation, and observation.

Option A mitochondria are sac-like structures.

B is not connected to the endoplasmic reticulum, which is connected to the outer membrane of the nucleus and is also connected to the cell membrane. For example, during the synthesis of secreted proteins, the Golgi apparatus encapsulates mature proteins in vesicles and transports them to the cell membrane, where the vesicle membrane fuses with the cell membrane and the proteins are excreted through exocytosis.

Electoral stimulus A mitochondria are sac-like structures.

B is not connected to the endoplasmic reticulum, which is connected to the outer membrane of the nucleus, and is also connected to the cell membrane. For example, in the synthesis of secreted proteins, the Golgi apparatus wraps mature proteins in vesicles and transports them to the cell membrane, where the vesicle membrane fuses with the cell membrane and the protein is excreted from the body through exocytosis.