How to use the pesticide residue rapid detector

1. Turn on the power switch on the back of the instrument, the instrument will display the boot screen, operate according to the prompts on the screen, and calibrate the zero point of the instrument before testing.

2. Reagent preparation.

Buffer: Add 1 packet of buffer to 500ml of distilled water or purified water, stir and dissolve to make phosphate buffer (, store at room temperature. Chromogenic agent:

Dissolve 1 bottle of chromogen with 25ml of buffer, take 100 l when using, and store in 4 refrigerators. Substrate: take 1 bottle of substrate and dissolve it with distilled water or purified water, take 100 L when using, and store it in 4 refrigerators; Or take 1 bottle of substrate and dissolve it in distilled or purified water, and store it in a refrigerator with 20 l, 4 when using.

Cholinesterase: The enzyme preparation does not need to be prepared, it can be taken directly, 100 L is taken when used, 4

Keep in the refrigerator. 3. Sample extraction Take 2g of fruit and vegetable samples (4g for tubers), cut the leafy vegetables into pieces about 25px square, take cross-sectional samples or take their skins for tubers, put them in a triangular bottle, add 10ml of buffer, shake for 1 2min, pour out the extract, stand for 2min, and wait for testing. If the extract is turbid or there are too many impurities, it can be filtered and then tested.

4. Test control test: add buffer to the reaction bottle, then add 100 l of enzyme solution and chromogenic agent respectively, mix well, add 100 l (or 20 l) of substrate after standing for 10min, shake well and immediately pour it into the cuvette, and put it into the measuring room channel of the instrument in time. Press the Control button, after the display delay is set for a second, the measurement time starts to count down, and when the timing is completed, the display displays the control absorbance increment (δ and prompts completion.

In the case of control testing, samples can be tested at the same time as other channels. Sample test: add the solution to be tested in the reaction bottle, then add 100 l of enzyme solution and chromogenic agent respectively, mix well, let the reaction stand for 10min, add 100 l (or 20 l) of substrate, shake well and pour it into the cuvette immediately, and put it into the measuring room channel of the instrument in time.

Press the Sample button, after the delay at the bottom of the display is set for a second, the measurement time starts to count down, and when the timer is finished, the display shows the sample absorbance increment (δ and inhibition rate, and indicates that it is qualified or exceeded. The data can be saved automatically, and if necessary, press the Print button to print.

1. How to use the pesticide residue detector:

Boot - do control test - extract samples - do sample test - print the inhibition rate of drug residues.

Second, the specific process:

1. Boot process.

Turn on the power supply, warm up for 8 seconds, and convert the computer output mode to the printer output mode.

2. Control test:

Take 1 cuvette, add 100ul of enzyme solution to the cuvette with a 100ul micropipette, and then add buffer with a 10ml pipette and shake well.

Use a 100ul micropipette with a replacement pipetting head, add 10ul chromogen, then use a 20ul micropipette, add 20ul substrate, seal with plastic wrap or a clean glass sheet and shake well.

Press "100%.""After the key, put the cuvette into the * channel on the top of the instrument, close the instrument cover, and press "Control.""key to display the control absorbance change value after 1 min.

Pesticide residue detector is a detection instrument developed by the principle of enzyme inhibition and photoelectric colorimetry.

Under certain conditions, organophosphorus and carbamate pesticides had inhibitory effects on the normal function of cholinesterase, and their inhibition rate was positively correlated with the concentration of pesticides. Under normal circumstances, the enzyme catalyzes the hydrolysis of the neuroconduction metabolite (acetylcholine), and the hydrolysate reacts with the chromogen to produce a yellow substance, and the absorbance change value with time at 412nm is measured with a spectrophotometer, and the inhibition rate can be determined, and whether the sample contains organophosphorus or carbamate pesticide residues can be judged by the inhibition rate.

It can realize on-site rapid detection of organophosphorus and carbamate pesticide residues. It can be widely used in the detection of pesticide residues in vegetables and fruits in product quality supervision and inspection, health and epidemic prevention, environmental protection, business administration, vegetable wholesale markets, vegetable production bases, supermarkets, shopping malls, pesticide residue monitoring systems and other departments.

Advantages of Pesticide Residue Detector:

1. Li Chao is fast.

Control and sample simultaneous testing can test up to 10 samples in as little as one minute.

2. Advanced scalability.

Microcomputer control and instrument detection can be carried out separately, and the operation is more flexible.

3. Easy to operate.

LCD large screen, Chinese prompt operation can be operated without professionals, the instrument is tested each time to self-check the effectiveness of the light source, no need to manually adjust with the light block. The instrument automatically determines whether the test data is qualified or not, replacing manual judgment, and the test results are more intuitive.

4. Low failure rate.

The instrument is powered on by the optical source inspection, and there is no need for manual adjustment of the light block of the same type of instrument in China, that is, there is no future maintenance, and the light source is more accurate.

5. The test data can be stored.

You can query the seller at any time, and save the information including, sample name, testing time, testing personnel, testing data, result judgment and other detailed data.

Summary. How to adjust the date of the pesticide residue rapid detector pesticide residue rapid detector time setting display time is set by the "set" key, use "+" or "-" to adjust the time, modify the time unit as minutes, its zui is 99 minutes, and the color development time accuracy is 1 second.

How to adjust the time of the pesticide residue rapid detector during the daily burial period, the display time is set by the "type liquid guess set" key, and the time is adjusted by "+" or "-", and the time unit is modified to minutes, which is 99 minutes, and the color development time accuracy is 1 second.

All testing instruments are the same.

How to modify this machine.

Click System Settings Pro.

The display time is set by the "set" button.

There is no pro-<> in the settings

Can you teach me how to do it?

Open the detection settings, oh dear.

Summary. There are some methods for the rapid detection of pesticide residues: 1. Chemical rapid detection method, mainly based on the redox reaction, the hydrolysate and the detection solution are discolored, and are used for the rapid detection of organophosphorus pesticides, but the sensitivity is low, the use is limited, and it is easy to be interfered with by reducing substances.

2. Immunity analysis methods, the key are radioimmunoassay and enzyme immunity analysis, the most common is enzyme-linked immunoassay (ELISA), according to the non-specific identification and fusion reflection of antigens and antibodies, artificial service antigens must be prepared for small content fertilizers, and immunity analysis can be carried out. 3. The enzyme inhibition method is the most mature and widely used rapid pesticide residue detection technology, which is mainly based on the specific inhibition reaction of organophosphorus and carbamate pesticides on acetylcholinelinease. 4. The face detection method is to use the sensitive reflection of living microorganisms to pesticide residues, such as feeding house flies with samples and observing the lethality rate to judge the amount of pesticide residues.

This method is simple to operate, but the judgment is not smooth, the accuracy is low, and the application field of chemical fertilizer is narrow.

Good. There are some methods for the rapid detection of pesticide residues: 1. Chemical rapid detection method, mainly based on the redox reaction, the hydrolysate and the detection solution are discolored, and are used for the rapid detection of organophosphorus pesticides, but the sensitivity is low, the use is limited, and it is easy to be interfered with by reducing substances.

2. Immunity analysis methods, the key are radioimmunoassay and enzyme immunity analysis, the most common is enzyme-linked immunoassay (ELISA), according to the non-specific identification and fusion reflection of antigens and antibodies, artificial service antigens must be prepared for small content fertilizers, and immunity analysis can be carried out. 3. The enzyme inhibition method is the most mature and widely used rapid pesticide residue detection technology, which is mainly based on the specific inhibition reaction of organophosphorus and carbamate pesticides on acetylcholinelinease. 4. The face detection method is to use the sensitive reflection of living microorganisms to pesticide residues, such as feeding house flies with samples and observing the lethality rate to judge the amount of pesticide residues.

This method is simple to operate, but the judgment is not smooth, the accuracy is low, and the application field of chemical fertilizer is narrow.

1. Blank production.

Extract milliliters of extract + 100 microliters of enzyme + 100 microliters of chromogen (incubate at 30 temperature for 15 minutes), then shake 100 microliters of substrate and pour it into the cuvette to put it into the first channel of the instrument (the blank must be operated in the first channel) Note: The blank value should be re-made after the instrument is restarted.

2. Sample testing.

Take 1g of the sample and cut it up, soak it in 5 ml of extract for 10 minutes (if there is a ** device, the ultrasonic time can be shortened to about 2 minutes) ......Take a milliliter of soaking solution + 100 microliters of enzyme + 100 microliters of chromogen (incubate at 30 temperature for 15 minutes), shake 100 microliters of substrate and pour it into a cuvette to put it into the instrument.

When the inhibition rate is 50%, it means that the organophosphorus or carbamate pesticides in vegetables exceed the national standard, and the sample with an inhibition rate of 50% needs to be tested more than twice.

Blank control test:

Step 1: Press the "Calibrate" key.

Step 2: Open the door cover of the instrument, put the cuvette containing the control solution into the designated channel, close the instrument cover, press the "blank" button, and the instrument will perform a blank detection countdown.

Step 3: After the blank control test is completed, the instrument will automatically display the blank sample control test result AO, and open the instrument cover to take out the control.

sample testing; Step 1: Press the "Calibrate" key.

Step 2: Open the instrument door cover, put the cuvette containing the sample liquid into the designated channel, close the instrument cover, press the "Detect" button, and the instrument will count down the sample detection.

Step 3: After the test, the instrument automatically displays the test results of the pesticide residue detection sample in the specified channel, the instrument automatically saves the control value and the test result, and opens the instrument cover to take out the sample.

Note] The inhibition rate of the sample is between 40% and 50% for the suspected pesticide residue exceeding the standard, if the inhibition rate is still greater than 50% after several repeats, it is the pesticide residue exceeding the standard, indicating that the pesticide residue of the tested sample may exceed the safety standard, and it is recommended to use meteorological chromatography and other instruments for further detection.

Test result printing: On the interface displaying the pesticide residue detector results, press the "Print" button to start printing.

The pesticide tester is mainly used for the rapid detection of organophosphorus and carbamate pesticides in fruits, vegetables, tea and grains. It is especially suitable for the rapid test of the field before the vegetable production base and the vegetable professional households, the on-site quick test of the farmer's wholesale market and distribution center, the supermarket, the vegetable field, the restaurant, the canteen, and the family fruit and vegetable processing before the quick test. It is suitable for the pollution-free supervision and testing center of agricultural products, the industrial and commercial department, the technical supervision bureau, the health and epidemic prevention station and other units to detect pesticide residues in the departments under their jurisdiction, and monitor the residual toxicity of fruits and vegetables on the market.

Use this instrument to first screen a large number of samples in and out of the country, and then use the instrument method to quantitatively analyze a small number of positive samples, which can greatly improve work efficiency, save a lot of manpower and material resources, and ensure food safety.

The pesticide residue detector is easy to use, and it uses enzyme inhibition, and the catalytic ability of cholinesterase to decompose substrates is positively correlated with its activity. The pesticides in vegetables inhibited the activity of cholinesterase, and the degree of inhibition was linearly related to the residual toxicity of pesticides. Therefore, the change of cholinesterase activity can be measured by measuring the rate change of catalytic reaction before and after the action of vegetable samples with cholinesterase by measuring the rate change of pesticide residue speed tester, so as to calculate the inhibition rate, and whether there is a high dose of organophosphorus pesticides in the sample can be judged through the inhibition rate.

The pesticide residue detection instrument FT-NC has a menu library of more than 100 vegetable names, which can be selected by clicking directly. And the name of the vegetable can be printed.

Detection channels: 8 detection channels, multiple samples can be tested at the same time, each sample is controlled by the program to work independently and will not interfere with each other.

The fast enzyme immunization kit, which I make antibodies, is the same as the food safety one.