How is cellulose in solution detected?

Because at room temperature, cellulose is insoluble in both water and general organic solvents, such as alcohol, ether, and acetone.

benzene, etc. It is also insoluble in dilute alkaline solutions. Therefore, bio-made powders are heated with acetic acid.

and nitric acid, in which case the intercellular material is dissolved.

Cellulose is also broken down into individual fibers, lignin.

Hemicellulose and other substances are also removed. Starch, pentose and other substances are hydrolyzed. After washing with water to remove impurities, the cellulose is potassium dichromate in the presence of sulfuric acid.

Oxidation to carbon dioxide.

and water. At room temperature, it is relatively stable because of the presence of hydrogen bonds between cellulose molecules. Cellulose is insoluble in water and ethanol.

Organic solvents such as ether.

The procedure for the detection of cellulose in solution is as follows:

1) Prepare the various solutions required, the ammonium ferrous sulfate solution is prepared within one week of use, and its titer k is determined on the day of use. Titrate 25 ml of potassium dichromate solution with this ammonium ferrous sulfate solution with m25

2) Weigh the naturally dried biomass powder with a value of n.

3) Put it into a centrifuge tube and add 5ml of nitric acid and acetic acid mixture.

4) Plug the centrifuge tube, boil in boiling water for 25min, and stir regularly.

5) Centrifuge, pour off the clear liquid, add distilled water to centrifuge and wash the precipitate, and wash three times.

6) Add 10ml of potassium dichromate solution and 8ml of concentrated sulfuric acid, stir well, put it in boiling water for 10min, and stir regularly.

7) Cool, pour into an Erlenmeyer flask, rinse the precipitate with a little distilled water, drop in 3 drops of test ferrous reagent, titrate with ammonium ferrous sulfate solution, use b ml, and end point from yellow through yellow-green to reddish-brown.

8) Add 8ml of concentrated sulfuric acid and 10ml of potassium dichromate solution to the ferrous ammonium sulfate solution separately titration, and use a ml.

9) The formula for calculating the content of cellulose in biomass: x% = sodium thiosulfate solution.

The experimental principle is as follows:

Bio-made powders are treated with a mixture of acetic and nitric acids when heated, in which case the intercellular material is dissolved, the cellulose is broken down into individual fibers, and lignin, hemicellulose and other substances are removed. Starch, pentose and other substances are hydrolyzed. After washing with water to remove impurities, the cellulose is oxidized to carbon dioxide and water by potassium dichromate in the presence of sulfuric acid.

c6h10o5 + 4k2cr2o7 + 16h2so4 = 6co2 + 4cr2(so4)3 + 4k2so4 + 21h2o

The excess potassium dichromate is titrated with ammonium ferrous sulfate solution, and then the same amount of potassium dichromate but not reacted with cellulose is titrated with ammonium ferrous sulfate, and the content of cellulose can be obtained according to the difference.

The products of cellulose hydrolysis in the presence of concentrated sulfuric acid are glucose and fructose, and cellulose can be detected by checking the presence of glucose, the hydrolysate of cellulose, according to the silver mirror reaction of glucose.

The items to be measured are boiled with neutral detergents, and the insoluble residue is neutral detergent fibers, mainly cell wall components, including hemicellulose, cellulose, mukaihe, and silicate. The plant-based feed is treated with acid detergents, and the remaining residue is acid detergent fibers, which include cellulose, lignin and silicate.

The residue of acid detergent fiber treated with 72% sulfuric acid is lignin and silicate, and the residue after 72% sulfuric acid treatment is subtracted from the value of acid detergent fiber to be the cellulose content of the feed. The residue treated with 72% sulfuric acid is ashed, and the part that escapes during the ashing process is the content of acidic washing lignin (ADL). Cellulose content analysis system based on the Vansoest principle.

Summary. To know whether cellulose is dissolved in it, it is necessary to conduct experimental testing. Infrared spectroscopy, ultraviolet spectroscopy, nuclear magnetic resonance and other technologies can be used to analyze the cellulose in the solution to determine whether the cellulose is dissolved into it.

To know whether cellulose is dissolved in it, it is necessary to conduct experimental testing. Infrared spectroscopy, purple mask megaspectroscopy, nuclear magnetic resonance and other technologies can be used to separate and precipitate the cellulose in the solution, so as to determine whether the cellulose is dissolved into it.

You've done a great job! Can you elaborate on that?

Cellulose is a major component of the cell wall of a plant, it is a polysaccharide that is insoluble in water and therefore cannot be dissolved in water. In order to dissolve cellulose, some special solvents such as alcohols, ethers, acids, etc. are used. Workaround:

1.Use special solvents such as Hetan: Special solvents such as alcohols, ethers, and acids can be used to dissolve cellulose, but pay attention to the proportion of solvents so as not to affect the properties of cellulose.

2.Use enzymes: Enzymes can be used to break down cellulose, but pay attention to the activity of the enzymes so as not to affect the properties of cellulose.

3.Use chemical methods: Chemical methods, such as hydrolysis, oxidation, etc., can be used to change the structure of cellulose so that it dissolves.

Personal Tips:1When dissolving cellulose, it is necessary to pay attention to the ratio of solvent so as not to affect the properties of cellulose.

2.When using enzymes to decompose cellulose, Chan Xiaotong should pay attention to the activity of enzymes, so as not to affect the properties of cellulose. 3.

When using chemical methods to change the structure of cellulose, it is necessary to pay attention to the reaction conditions so as not to affect the properties of cellulose.

Cellulase is generally found in bacteria or fungi, and some herbivores can also digest cellulose.

Substances containing cellulase can dissolve cellulose and are generally harmless to the body.

In addition, the following chemical reagents can also dissolve cellulose, but they are harmful to the body.

1. Curamia solution, generally use cupro solution to dissolve cellulose to determine the viscosity of cellulose and calculate the degree of polymerization of cellulose.

2. Carbon disulfide, generally use carbon disulfide to dissolve cellulose to prepare viscose fiber, that is, regenerated fiber, or tencel.

3. Concentrated alkali + urea.

Cellulose is not fiber, the two are two overviewed sedan chairs. Cellulose uses a cellulose analyzer to determine the content of the sail cavity, which is generally determined to contain crude fiber, and dietary cellulose is also determined in food.

Hello, cellulose is a polymer sugar, there are many types, since the instrument is used to measure its content, it should be available for both acidic and alkaline, and the measurement can be completed according to the instructions for use.

Cellulose is acidic and alkaline because of the different groups attached to it, and it is conceivable that the acidity and alkalinity of this polymer is very weak according to the pH value, so it should be okay to regard it as neutral.

Hemicellulose is a mixture of several different types of polysaccharides that are tightly bound to cellulose in plant cell walls, including xylan, xyloglucan, and galactomannan, among others.

Neutral cellulose refers to cellulose that is neither significantly acidic nor significantly alkaline.