Chemiluminescence immunoassay analyzer in China has several in production What is the situation

There are more than 10 of them.

Now the domestic ones are:1Shenzhen's new industry (semi-automatic is half-done, and recently it is rising due to fully automatic listing).

2.Beijing Yuande (semi-automatic equipment has a large number of domestic equipment, and fully automatic equipment has also been listed in 12 years). 3.

Beijing Bei Aikang (a model of magnetic particle luminescence method in China, has been acquired by Yuande). 4.Beijing Kemei Dongya (semi-automatic at the midstream level, fully automatic is just a combination of automatic sampling and semi-automatic luminescence, not fully automatic in the real sense, the current market is very general) 5

Xinbo (the representative of the domestic time-resolved luminescence "there is also a Guangzhou Fenghua who does time-solving, but the market performance is not as good as Xinbo", the domestic market of the hepatitis B project is doing very well, and it is currently being acquired by PE). 6.Zhengzhou Antu (Antu has a general foundation in biotechnology, but its ability to do the market is first-class in the country!)

The level of equipment is average, but the parameters are very good. At present, the market is generally doing well, and fully automatic will be launched soon). 7.

Weihai Weigao (semi-automatic is very bad, recently listed 200 speed fully automatic, the equipment is very large and beautiful, but the reagent situation and actual use is not clear, the overall market performance is currently average). 8.Beijing Tiger Kexin, a veteran luminescent manufacturer (its reagent batch number is very complete, and there are even batch numbers but can't make reagents!)

The level of technology is average, the market is chaotic, and it is getting lower and lower). 9.Sichuan Mike (the newly listed fully automatic, but the reagent variety is very small!)

Haven't seen users yet). 10.In addition, there are some small manufacturers such as Beijing Dacheng and Hebei Compson (only production equipment)!

Nanjing base egg, Shenzhen new industry and other manufacturers.

Two categories 1Chemiluminescence labeled immunoassay.

Chemiluminescence labeled immunoassay, also known as chemiluminescence immunoassay (CLIA), is an immunoassay method that directly labels antigens or antibodies with chemiluminescent agents. Commonly used chemiluminescent substances for labeling are acridine ester esters (AE), which are effective luminescence markers [3] which emit light by initiating a luminescence reagent (N AOH2H2O2), and the intense direct luminescence is completed in one second, which is a rapid scintillation (see Figure 1). Acridine ester is used as a marker in immunoassays, and its chemical reaction is simple, rapid, and does not require catalysts. The competition method was used for the detection of small molecule antigens, and the sandwich method was used for the principle of large molecule resistance, with less non-specific binding and low background. Binding to macromolecules does not reduce the amount of light generated, thus increasing sensitivity.

2.Luminescent enzyme immunoassay.

From the perspective of labeled immunoassay, chem ilum inescen t enzym e imm unoassay (CL e IA) should belong to enzyme immunoassay, but the substrate of the enzymatic reaction is a luminescent agent, and the operation steps are exactly the same as those of enzyme immunoassay [5]: the enzyme labels the bioactive substance (such as enzyme-labeled antigen or antibody) for the immune reaction, and the enzyme on the immune response complex acts on the luminescent substrate and emits light under the action of the signaling reagent. Luminescence assays are performed with a luminescence signal meter. At present, the commonly used labeling enzymes are horseradish peroxidase (HRP) and alkaline phosphatase (ALP), which have their own luminescent substrates.

Chemiluminescence immunoassay analyzer is a kind of immunoassay analyzer that directly labels antibodies with luminescent agent, and the detection sensitivity and automation are very high, so its accuracy evaluation can only use standard biochemical reagents. Standard biochemical reagents: immunoQC serum with uncertainty of 0 3 (k=2);

Calibration equipment, reagents, and working environment:

Pipette: 50ml, grade A; Measuring cup: 25ml; Indexing pipette:

0 25ML, Class A: Electronic AC Voltage Regulator: AC220V, 1KW; Mixer.

The temperature in the calibration chamber is (20 5): the relative humidity in the calibration chamber is not 80; The power supply voltage needs to be regulated by the electronic AC voltage regulator: there are good dust-proof measures in the calibration room

Keep away from the interference of strong electromagnetic fields.

【Shanghai Bajiu】Reminder: The microplate reader is just a simple detection instrument, and there should be no doubt about this. As for the chemiluminescence immunoassay analyzer, it depends on the situation, and the explanation upstairs is a "fully automatic chemiluminescence instrument" or "system"; It can also be used as a simple testing instrument, without a pretreatment system, sample reaction system, etc., at this time it is a simple testing instrument.

In principle, the microplate reader is to detect the absorbance value of the liquid in the microplate plate, obtain an OD value and perform qualitative or semi-quantitative analysis to achieve the purpose of detection. Its advantages are that the instrument is simple, inexpensive and easy to operate; The disadvantages are low sensitivity, narrow detection range, and difficulty in automation.

Chemiluminescence immunoassay analyzer is the light signal generated by chemiluminescence reaction (enzymatic luminescence or direct luminescence) through the photomultiplier tube for signal conversion and waiting until the corresponding signal value, expressed by RLU (relative light unit), in order to achieve quantitative or qualitative detection purposes. The advantages and disadvantages are almost the opposite of the microplate reader.

Summary. Hello, the top ten brands of immunoluminescence analyzer are ranked as follows:1

Bayer 2GLP (Perkinelmer) 3Daan Gene 4

Philips 5Magbio Genomics 6Randox 7

Emerson 8Sinocare 9.Applied Biosystems 10

Genentex Please note that the above rankings are not official rankings and are for reference only. Choosing the right immunoluminescence analyzer for you is based on your needs and budget.

Hello, the top ten brands of immunoluminescence analyzer are ranked as follows:1Bayer 2

GLP (Perkinelmer) 3Daan Gene 4Philips 5

Magbio Genomics 6Randox 7Emerson sedan sells 8

Sinocare 9.Applied Biosystems 10Genentex Please note that the above rankings are not official rankings and are for reference only.

Choosing the right immunoluminescence analyzer for you requires a comprehensive consideration based on your needs and budget.

When choosing an immunoluminescence analyzer, the following factors need to be considered:1Measuring range:

Different immunoluminescence analyzers can be used for different measurements, so you need to determine the required measurement range according to your needs. 2.Sensitivity:

The higher the sensitivity, the wider the range of concentrations that can be detected, which also means the higher the accuracy. 3.Detection speed:

The speed of detection can affect productivity. Some fully automated immunoluminescence analyzers can test a large number of samples in a very short and slippery time. 4.

Ease of use: Ease of use is one of the most important factors. Some immunoluminescence analyzers have a user-friendly interface and easy-to-understand procedures, which make the testing process easier.

5.After-sales service: After-sales service and technical support are one of the important options worth considering when using a device for a long time.

Ensuring that you have access to timely and effective after-sales support and repair services will give you a great sense of peace of mind. Based on the above factors, you can choose the right brand and model of immunoluminescence analyzer according to your actual needs.

Basic Information:

Product Name: Automatic chemiluminescence immunoassay analyzer.

Product performance structure and composition: This product is composed of a fully automatic chemiluminescence immunoassay analyzer. It is mainly composed of reagent area, sample area, reaction test tube sampling area, and reaction waste liquid area.

Scope of application: For the analysis of analytes in blood samples in the laboratory.

Introduction:Immunoassay has gone through different periods such as radioimmunoassay, fluorescence immunoassay, enzyme labeled immunoassay, etc., and automatic chemiluminescence immunoassay is a new stage in the development of immunoassay, which has been widely recognized as environmentally friendly, fast and accurate. Therefore, fully automated chemiluminescence immunoassay is the only way to the perfect state of immunoassay.

Working principle: Chemiluminescence immunoassay analyzer is a medical testing instrument that can detect the patient's serum and perform immunoassay on the human body. Quantified patient serum and horseradish peroxide (HRP) were added to a solid-coated white opaque microplate with antibodies, and the molecule to be tested in the serum specifically bound to the horseradish peroxidase conjugate and the antibody on the solid support. Separate and wash unreacted free components.

Then, luminol luminescent substrate is added, and the free energy released by the chemical reaction is used to excite the intermediate, from the ground state back to the excited state, and the energy is released in the form of photons. At this point, the microplate is placed in the analyzer, and the photon counter reads out the number of photons in each well in turn through the three-dimensional transmission system inside the instrument. The concentration of the molecule to be measured in the sample was quantified according to the mathematical model established by the standard.

Finally, a data report is printed to aid in clinical diagnosis.

There is also Beijing Lianzhong Tech, which is also said to be quite good.

abbott：architect i1000/i2000/i4000 sr

axsym、axsym plus

Siemens: (formerly Bayer) Advia Centaur CP, Advia Centaur XP

Original DPC) Immulite 2000

beckman coulter：access/access ii

diasorin：liaison

ortho clinical diagnostics：vitros eci immunodiagnostic system

roche（eclia）：elecsys2010、cobas e

Weihai Weigao Biotechnology: fully automatic autolumis2000

Shenzhen New Industrial Bio: Maglumi 2000 Plus

Shanghai Lanyi: Fully automatic immunoluminescence system AIA-2000

Zhengzhou Antu Biological: Autolumo A2000

The specific data of market share can not be given, Roche is the largest in chemiluminescence immune products, Abbott is almost tied with Siemens for second, and the rest are small fish and shrimp.

Give points!!

Many of the questions you asked are in my memory, but I haven't summarized them. If you really want to follow the summary you asked, I guess I'll have to write it for a week. But I really don't have the time, I'm really sorry.

You'd better be able to ask one by one. I ask by a manufacturer. In addition, I am familiar with the two provincial markets of Hubei and Henan, and I know a rough idea of what I know nationwide.

Hello, because the immunoluminescence analyzer is a high-end medical equipment, there are many brands and models in the domestic market, and there are great differences in performance, quality and scope of application between different brands. The following is a list of some immunoluminescence analyzer brands (in alphabetical order) in the current domestic market:1

Abbott (Abbott) 2Beckman Coulter (Beckman Coulter) 3Diasorin (Diasolin) 4

f.Hoffmann-La Roche (Roche Diagnostics) 5Luminex (road star) 6

Maglumi (Maibi Liyu Gelong) 7Mindray (Mindray) 8Siemens Healthineers 9

SNIBE Diagnostic (SINBON Biotech) 10TOSOH Bioscience (Tohoku TOSOH) should note that the above are only some brands, and there is no specific ranking, which does not mean that the performance and quality of other brands are not good. In the actual purchase and use process, the selection should be made according to the actual demand, budget and performance requirements and other factors, so as to choose the most suitable means of the product.

Hello. Chemiluminescence labeled immunoassay, also known as chemiluminescence immunoassay (CLIA), is an immunoassay method that directly labels antigens or antibodies with chemiluminescent agents. The chemiluminescence immunoassay analyzer consists of two parts, namely the immune response system and the chemiluminescence analysis system.

The chemiluminescence analysis system uses chemiluminescent substances to form an excited state intermediate through the catalysis of catalyst and oxidation of oxidant, and when the excited state intermediate returns to a stable ground state, photons (hm) are emitted at the same time, and the optical quantum yield is measured by luminescence signal measuring instruments. The immune response system is to label a luminescent substance (an excited state intermediate under the excitation of a reaction agent) directly on an antigen (chemiluminescence immunoassay) or antibody (immunochemiluminescence analysis), or an enzyme acting on a luminescent substrate.

Microplate reader, also known as enzyme-linked immunosorbent detector, is a special instrument for enzyme-linked immunosorbent assay. It can be simply divided into two categories: semi-automatic and fully automatic, but its working principle is basically the same, and its core is a colorimeter, that is, the colorimetric method is used to analyze the content of antigens or antibodies. ELISA determination generally requires the final volume of the test solution to be less than 250U L, which cannot be completed with a general photoelectric colorimeter, so there are special requirements for the photoelectric colorimeter in the microplate reader.