What is the method of saliva sampling for genetic testing?

Saliva sampling and testing method is more convenient, fast and easy to operate. The process is as follows:

1. Do not eat, drink, smoke, drink or chew gum within 30 minutes before saliva collection, and rinse your mouth with drinking water.

2. Take out the sampling tube, open the funnel cover, and collect saliva into the sampling tube through the funnel until the position indicated on the label on the sampling tube.

3. Close the funnel lid and let the preservation solution in the funnel lid fully flow to the collection tube.

4. Unscrew the funnel, cover with a small lid, and shake it upside down to make the saliva and the preservation solution fully mixed.

5. Put the sampling tube and the filled information back in the box and send it back to the genetic testing center.

Gene is a functional fragment of DNA molecule, the basic unit of genetic information, and the most basic factor that determines all biological species. Genes determine human life, aging, sickness and death, are the cause of health, beauty and longevity, and are the manipulators and regulators of life. Therefore, if there is life, there will be genes, and the form of existence and decay of all life is determined by genes, including your appearance, height, weight, skin color, personality, etc., which are inseparable from genes.

Genetic testing is a technique that uses blood, other body fluids, or cells to detect DNA.

Genetic testing is a technology that detects DNA through blood, other body fluids or cells, which is to take the oral mucosal cells or other tissue cells shed by the tested person, expand their genetic information, and detect the DNA molecular information in the cells of the tested person through specific equipment, predict the risk of disease in the body, and analyze the various genes it contains, so that people can understand their own genetic information, so as to avoid or delay the occurrence of diseases by improving their living environment and living habits.

Genetic testing can be used to diagnose diseases and can also be used to determine the risk of disease**. Disease diagnosis is the use of genetic testing technology to detect mutated genes that cause hereditary diseases. At present, the most widely used genetic testing is the detection of neonatal hereditary diseases, the diagnosis of genetic diseases and the auxiliary diagnosis of some common diseases.

There are currently more than 1,000 genetic diseases that can be diagnosed by genetic testing.

**Genetic testing is the use of genetic testing technology to detect the risk of disease before it occurs, and to prevent it early or take effective interventions. There are already more than 20 diseases that can be treated with genetic testing**.

In the test, the person's genes are extracted from the blood or other cells. Then, primers and PCR technology that can identify genes that may have mutations are used to replicate these genes many times, and mutant gene probe methods, enzyme digestion methods, gene sequence detection methods, etc. with special markers are used to determine whether there are mutations or sensitive genotypes in these genes.

Genetic testing: It refers to a technology that detects DNA molecules in the cells of the tested person through gene chips and other methods, and analyzes the pathogenic genes and disease susceptibility genes contained in the tested person.

At present, the main methods of genetic detection are: fluorescence quantitative PCR, gene chip, liquid biochip and microfluidic technology.

Saliva collects high-quality and high-yield DNA and stores it at room temperature for a long time.

Saliva collector.

A 1ml saliva sample is collected in a test tube and mixed with a patented stabilized liquid to provide auxiliary collection, and the saliva sample can be stored stably for several years at room temperature

Do not eat, drink, smoke or chew gum for 30 minutes before the saliva sample is taken. Do not peel off the plastic film on the funnel lid, relax your cheeks and gently rub for 30 seconds to produce saliva before spitting. Saliva DNA Sample Collection Tube - Collection Method:

1. Gently spit into the oral endocrine saliva into the oval collection mouth of the saliva collector, and pay attention to avoid excessive foam when collecting saliva.

2. Collect saliva until the layered interface between the saliva in the collection tube and the upper layer of foam reaches the 2 ml scale mark.

3. Evenly buckle the lid of the collector filled with the preservation solution to the oval collection port, there is a slight clicker sound when the deposit is detained, and the preservation solution flows into the collection tube evenly, indicating that the lid has been buckled, otherwise, you need to gently open the lid of the collector, and then close it evenly again until the preservation solution flows into the collection tube evenly.

4. Gently shake the saliva collector so that the preservation solution flows into the collection tube more completely.

5. Confirm that the layered interface between the liquid and the upper layer of foam in the collection tube reaches the 4ml scale shown in the tube.

6. Confirm that the collection is complete.

Note: First of all, DNA is extracted through the exfoliated cells contained in the saliva, not the saliva itself. Therefore, when collecting saliva samples, you should use your tongue to scrape your upper and lower jaws as many times as possible, and you can also use your teeth to scrape your tongue slightly to ensure the number of cells shed.

Ensure that you have not eaten, drunk, smoked and other behaviors that affect the success rate of sampling in the past 30 minutes, and it is recommended to take samples first thing when you get up in the morning to ensure the success rate of sampling. It is important to note that you do not rinse your mouth with mouthwash before sampling, and it is not recommended to wait until after rinsing.

Saliva sampling method gene sample collection process:

1. Oral hygiene: The examinee is required to rinse his mouth with water, and he or she is not allowed to eat, drink, smoke, drink or chew gum within half an hour.

2. Pre-harvest preparation: Wear disposable medical gloves (using hospital standard wearing methods and procedures), open the collection box and prepare for sampling. Remove the tube from the box, remove the plug cap, and insert the tube into the funnel.

3. Take saliva: collect saliva into the sampling tube through the sampling funnel until the position indicated on the label on the sampling tube. (Note: To ensure that the actual amount of saliva reaches the scale mark, the upper layer of foam is not included, and the saliva sampling process must be completed within half an hour.) ）

4. Save the sample: take out the lid of the cup from the box, the lid contains the fixative solution, and screw the lid of the cup in the clockwise direction of the sampling funnel. After confirming that the lid of the cup is tightened, invert it 10 times to mix the saliva and fixative solution thoroughly, keep the lid of the cup on top, and place the sampling tube vertically for 5 minutes.

Keep the sampling tube vertical, pull out the sampling funnel, cover the sampling tube tightly with the plug cap, and mix upside down 10 times. (Note: The fixative solution in the lid of the cup should be clear, colorless and transparent, if it is cloudy, please discard it.)

Please do not tear the plastic film inside the lid with your hands. ）

5. Mailing samples: discard the sampling funnel and sampling cup lid, put the sampling tube and the completed information into the paper bag of the ziplock bag, and seal the ziplock bag tightly and send it to the test.

6.Zhongyuan Concord Gene is doing it!

The so-called DNA identification saliva sample is actually oral mucosal cells, which need to be paid attention to when collecting:

1.Rinse your mouth with water before collection, then prepare a piece of white paper, take a medical sterilized cotton swab, stick it into the mouth, scrape it back and forth more than a dozen times near the inside of the cheek, and rotate the cotton swab from time to time to fully contact the oral mucosa.

2.Remove the cotton swab and place it on white paper to dry naturally.

3.In the same way, scrape each cheek separately, obtain two cotton swabs, dry them naturally, mark them, wrap them in white paper, and put them in an envelope.

4.Repeat the step to take the oral mucosal epithelial cells of another person. Each person's sample is placed in a separate envelope and mailed by express mail as soon as possible.

Bethune Genetic Medical Center, PLA Professional Paternity Testing Center.

Cups, beverage bottles, toothbrushes, etc., from which the person being identified drank water.

A cotton swab rubbed in the mouth will do.

The Ala 88 test is the saliva that is collected, and the saliva is a little better because the saliva contains more DNA than the blood.