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Because the composition of the medium in tissue culture is determined by different plant species, it is necessary to prepare targeted medium in order to achieve better results in tissue culture, and these are to be explored and studied. Some cherished plants due to a variety of special reasons, their survival rate is very low, and some plants must also survive in a specific environment and climate (symbiotic bacteria, cooperation with animals, cooperation with other plants, some special trace elements, a certain air humidity, etc. are all limiting conditions), once transplanted to other places it is difficult to survive, so the requirements of environmental conditions have increased the difficulty of tissue culture. The use of tissue culture to propagate endangered plants is not unused, but scientists have been experimenting with it and several have been successful, as if Metasequoia metasequoia was once a good example of success with tissue culture.
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Theoretically, all plants can do tissue culture, but many cherished plants cannot induce differentiation, so just cultivating a bunch of cells is useless. Not all plants can be promoted with this method, the tissue culture conditions of many rare plants are still unknown, and at present, China can only do some tissue culture of Chinese herbal medicines, you are a high school student, right? It seems that you still don't know a lot of tricky problems in tissue culture!
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Endangered rare plants that have adapted to the environment are currently reproducing and growing according to the idea of LZ, and should have achieved good results.
For climate, humidity, altitude and other high requirements, it is difficult to do it manually.
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I have also considered the landlord's question and hope that the professional will answer it.
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1.Aseptic operation techniques should be strictly implemented at all stages of plant tissue culture, and in order to prevent contamination, plant tissues must first be sterilized (sterilized), and various utensils and culture media are commonly used (autoclave method).
2.The callus differentiates into roots and shoots, and from the buds to leaves and stems, a process that requires light for photosynthesis after the young leaves are grown.
3.If plant apical cells are selected for tissue culture, the plant leaves in the tube are (green) because (apical cells contain genes associated with chloroplast formation).
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1. Sterilization and disinfection treatment of the sterilization pot.
2.Plants absorb sunlight in order to synthesize the organic matter they need.
3. Plant apical cells that do not carry viruses are new cells.
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