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When the pH is greater than the isoelectric point, the carboxyl group.
Ionization predominates amino acids.
When the ions are less than the isoelectric point, the amino group protonation is dominant, and the amino acid ions are positively charged.
Amino acids contain two main functional groups, amino and carboxy.
Both groups can be ionized as follows:
Carboxyl-cooh <—coo- +h+
The amino-NH2+H2O<-NH3+ +OH-two ionization reaction makes the amino acids charged opposite, because both can occur, so when the two are equally ionized, the amino acids are neutral, and the pH value of the solution at this time.
This is called the isoelectric point.
Because the structure of amino acids is different, their own ionization tendency is also different when they are not affected by the outside world. Each amino acid has an isoelectric point: in a solution of a certain pH, the amino acid or protein dissociates into cations and anions with equal tendency or degree, becoming facultative ions and electrically neutral.
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The two main functional groups of amino acids.
Carboxyl -Cooh <===>-CoO- +H+Amino -NH2+H2O<===>-NH3+ +OH- Because the structure of amino acids is different, their own ionization tendency is also different when they are not affected by the outside world.
Each amino acid has an isoelectric point (PI): in a solution of a certain pH, an amino acid or protein dissociates into cations and anions with equal tendencies or degrees, becoming facultative ions and electrically neutral.
When pH Pi, carboxyl ionization is dominant and amino acid ions are negatively charged.
When phWhen < pi, amino protonation is dominant, and amino acid ions are positively charged.
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There are several possibilities:1. In terms of polarity, the polarity of the solvent increases, the RF of the substance increases, and the acid and base increase, that is, the polarity of the solvent increases. Thus improving the RF of the substance.
Second, the pH value affects amino acids.
The degree of dissociation is related to pH, the larger the degree of dissociation, the stronger the polarity, and the more polar the substance is biased towards one of the polarities when the two phases are divided, the greater the degree of dissociation of amino acids, the smaller the rf. (pH has little effect on neutral amino acids) you add alkali to the solvent system, for acidic amino acids, carboxyl bending.
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Amino acids are species with both amino and carboxyl groups, in the aqueous solution, the carboxyl group loses hydrogen ions and is negatively charged, while the amino group gets hydrogen ions positively charged, because the acidity of the carboxyl group and the alkalinity of the amino group are not the same, so the amino acids are often charged as a whole. Adjusting the pH value of the solution can change the ionization status of the two, and at a certain point, the negative charge of the carboxyl group is the same as the positive charge of the amino group, and the amino acid is uncharged as a whole, and the pH value of this point is the isoelectric point of the amino acid.
The ionization constant of -cooh is Ka1
The ionization constant of NH3+ is Ka2, then the pH of the isoelectric point is.
In the illustration of the isoelectric point of a little amino acid.
It's the pH value.
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Due to amino acids.
Contains acidic carboxyl groups.
and alkaline amino groups, so are both acids and bases, and are amphoteric electrolytes.
The charge of an amino acid depends on the pH value of the environment, and changing the pH value can make the amino acid positively or negatively charged, or it can be in an equal number of positive and negative charges, i.e., a zwitterionic state with zero net charge.
Metal oxides generally undergo chemical reactions when dissolved in water, and even if the solution can conduct electricity, it cannot be said that metal oxides are electrolytes. Therefore, it is generally judged by whether it can conduct electricity in the molten state.
Ionic compounds and partially covalent compounds.
It is a strong electrolyte.
It depends on the substance.
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