Definition of recombinant protein, what is recombinant protein

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It is a synthetic protein. After knowing the sequence of the gene encoding the protein, the gene is manually cloned into the plasmid. The plasmid is then transfected with plasmids such as E. coli.

In this way, E. coli becomes a protein expression factory. The expressed protein is then isolated and purified, which is a recombinant protein.

Why is it called reorganization? This is because the genes in their natural state are recombined into the express body (the plasmid of E. coli).

Albumin (ALB) is synthesized by liver parenchymal cells, and has a half-life of about 15-19 days in plasma, which is the most abundant protein in plasma, accounting for 40%-60% of total plasma protein. Although its synthesis rate is affected by the protein content in food, it is mainly regulated by the level of albumin in plasma, which is not stored in hepatocytes and contains trace amounts of albumin in all extracellular fluids. Regarding the filtration of albumin in the glomeruli, it is generally believed that under normal circumstances, the amount of albumin is very small, about the albumin in the plasma, according to this calculation, the albumin excreted from the glomerular filtrate can be reached every day, which is 30-40 times the amount of protein excreted in the final urine, and it can be seen that most of the albumin in the filtrate can be reabsorbed by the renal tubules.

Experiments have confirmed that albumin is absorbed in the proximal convoluted tubule, and in the tubular cells, it is degraded into small molecule fragments by hydrolase enzymes in lysosomes and enters the blood circulation. Albumin can be taken up by intracellular endocytosis in different tissues, and its amino acids can be used for tissue repair.

The molecular structure of albumin was elucidated in 1975 as a single-chain polypeptide containing 585 amino acid residues, with a molecular weight of 66458, 17 disulfide bonds in the molecule, and no sugar components. In the environment of body fluids, albumin is a negative ion, and each molecule can carry more than 200 negative charges. It is the main carrier in plasma, and many poorly water-soluble substances can be transported by binding to albumin.

These substances include bilirubin, long-chain fatty acids (which can bind 4-6 molecules per molecule), bile salts, prostaglandins, steroid hormones, metal ions (e.g., Cu2+, Ni2+, Ca2+), drugs (e.g., aspirin, penicillin, etc.).

Active hormones or drugs, when bound to albumin, can not show their activity, but as their storage form, because of the reversibility of this binding and in dynamic equilibrium, so it is of great significance in regulating the metabolism of these hormones and drugs.

Albumin 1, albumin purification.

2. Purification method of albumin.

3. Affinity production process of albumin and antibody.

4. A manufacturing method for human serum albumin including a heat treatment step 5. A method for preparing protein from animal blood by precipitation method.

Method for preparing protein from animal blood by lake method A method for preparing protein from animal blood by precipitation method. With animal serum (slurry), ethanol was added, stirred, filtered, the filtrate was taken, the precipitant and stabilizer were added, albumin and gamma globulin were separated, and after the precipitator was removed, the dialysis was balanced to obtain albumin and gamma globulin. The yields of albumin and gamma globulin in this method were and the total content of albumin and gamma globulin of the two proteins were exceeded, respectively

The protein prepared by the method of the present invention can be used for the production of oral liquid, tablet, capsule.

Human serum albumin is the most abundant protein in human plasma, with many important physiological properties and a wide range of uses.

The production of recombinant proteins is a protein obtained by applying recombinant DNA or recombinant RNA technology. At present, the production of recombinant proteins in vitro mainly includes four major systems: prokaryotic protein expression, mammalian cell protein expression, yeast protein expression and insect cell protein expression.

Proteins produced by blindness differ in activity and application methods. According to their own downstream applications, select the appropriate protein table and empty the system to improve the expression success rate.

Protein recombination must first be hydrolyzed into amino acids, and there are many types of proteins because of its large number, complex structure, and ever-changing spatial structure. The basic amino acids are only 20, so when they are recombined, they can become completely different things, controlling metabolic processes that are completely unrelated to what they were before.

Genetic recombination only controls the expression of the same trait, but it does not change the basic situation of the trait. For example, if the gene that controls eye color is recombined, the parent may be black and the offspring blue.

But the gene that never controls eye color becomes the gene that controls the ear when recombined.

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If you want to buy an active protein for binding experiments, should I buy recombinant protein or natural protein?

See, here's what the focus interview is about:

It was in this context that Dr. Xie Liangzhi, who returned from Harvard in the United States in 2003, brought his own technology to Yizhuang and started his own business. After nearly a decade of development, he and his team have also become the biggest beneficiaries of the transformation of the development zone. Here, courier companies ship their products all over the world every day.

And their product is very special, it is an active protein substance, it is a scientific research tool for the development of recombinant proteins, which is very valuable and valuable.

From the perspective of R&D and manufacturing products, the added value of Sino Biological's products is much higher than that of the high-end manufacturing industries with foreign investment background introduced ten years ago. As far as the entire development zone is concerned, driven by innovation, in the past ten years after the transformation, its development quality and efficiency and other hard indicators have ranked among the top in the country.

I have never heard of a ...... of recombinant protein being sold in a bottle